37906268

Correction for: ASXL1 promotes adrenocortical carcinoma and is associated with chemoresistance to EDP regimen

Liang Wang1,*, Yinfeng Lyu2 ;* , Yuqing Li2, Kunping Li2, Hui Wen2, Chenchen Feng2, Ning Li3

1Department of Urology, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China

2Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China

3Department of Urology, Fourth Affiliated Hospital of China Medical University, Shenyang 100032, Liaoning Province, P.R. China

*Equal contribution

Correspondence to: Chenchen Feng, Ning Li; email: drfengchenchen@163.com, https://orcid.org/0000-0002-1854-356X; air- nick@hotmail.com, https://orcid.org/0000-0003-3420-0206

Keywords: adrenocortical carcinoma, ASXL1, chemoresistance

Original article: Aging (Albany NY) 2021; 13: pp 22286-22297

PMID: 34536950

PMCID: PMC8507286

doi: 10.18632/aging.203534

This article has been corrected: The authors found an error in Figure 3B. During assembly of the figure, incorrect images of colonies formed by ACC cell lines in which ASXL1 was silenced by shRNA#1 (KD1) were used. The authors prepared a new Figure 3 using representative images from the original experiments. This correction has no impact on the main conclusion. The authors would like to apologize for any inconvenience caused.

New Figure 3 is presented below.

Figure 3. Silencing of ASXL1 decreased fitness of adrenocortical carcinoma (ACC) cells. (A) Cell count detected using CCK-8 in ACC cell lines with ASXL1-knockdown (KD) by shRNA#1 and shRNA#2 (KD1 and KD2) or negative control (NC); (B) Colony formation in ACC cell lines with ASXL1 silencing or control; Flow cytometry used to detect (C) cell cycle profile and (D) apoptosis in ACC cells with ASXL1-KD or NC; (E) Transwell assays used to detect cell invasion with Matrigel in ACC cells with ASXL1-KD or NC, captured at 100x; (F) Wound healing assay in ACC cells with ASXL1-KD or NC ( ** P < 0.01).

A

H295R

B

H295R

12.

11-

SW-13

100-

100

SW-13

11

10

NC

10

Cell Count/Fold

9

NC

ASXL1-KD1

Cell Count/Fold

Colony formation

80

NC

9

8

ASXL1-KD1

Colony formation

80

NC

ASXL1-KD2

7

6

ASXL1-KD2

60

60

**

S

4.

**

40

40

**

**

3

**

KD1

KD1

2

2

20

20

1

1.

0

0

0

0

day1 day2 day3 day4 day5

day1 day2 day3 day4 day5

NC

KD1

NC

KD1

ASXL1

ASXL1

C

H295R

D

H295R

E

H295R

SW-13

60-

Ig

NC

150

I

ASXL1

A

NC

ASXL1

2.75-

**

O

2.50

Cell invasion (% of NC)

NC

Percentage (%)

50

**

**

NC

KD

:

ASXL1

KD1

% Apoptosis

2.25

NC

KD

100

T

40

2.00

T

1.75

&

30

1.50

KD1

T

KD1

1.25

50

1.00

**

**

**

20

0.75

0.50

T

10

0.25

0

G1

S

G2/M

-

Early

Late

H295R

SW-13

APC

SW-13

1

NC

SW-13

H295R

65

NC

60

T

1-

9.

F

ns

1.

80-

ASXL1

Percentage (%)

55

**

8

50

ASXL1

**

I

PFKFB4

Wound healing (%)

NC

Oh

2

45

NC

% Apoptosis

7

NC

**

KD

60

KD1

T

40.

KD

6

ã

T

35

30

KD1

KD1

5

40

25

20

4.

20

**

**

OX

15.

T

T

5h

10

3

T

5

G1

S

G2/M

2

0

Early

Late

APC

H295R

SW13