MEDICAL SCIENCE MONITOR

Received:2022.07.11
Accepted:2022.07.11
Available online:2022.07.18
Published:2022.07.28

ERRATUM

Errate: Identification of NDRG Family Member 4 (NDRG4) and CDC28 Protein Kinase Regulatory Subunit 2 (CKS2) as Key Prognostic Genes in Adrenocortical Carcinoma by Transcriptomic Analysis

Zhengqing Yang Hui Cheng Yazhou Zhang Yan Zhou

Department of Urology, Shanghai Yangsi Hospital, Shanghai, PR China

Corresponding Author: Yan Zhou, e-mail: zhou.yanys@hotmail.com

The authors would like to correct the following errors:

The original published paper had the wrong labels for Figure 3F. Figure 3 should have been as follows:

A

Sample denrogram and trait heatmap

B

Scale independence

D

7 8 91011121914151817161820

1.1

80

Scale free topology model fit, signed R2

0.8

3

0.9

75

0.6

Height

GSN1972939

GSM197221

GSM1972924

GSM1972333

0.7

2

70

0.4

0.5-

MEnanejow hibe1

GSM11900927

GSM11972931

GSM1972985

0.2

MEbrovin?

MEbrovin4

MEdarkgre

MEgreen

MEmagenta

65

MEdarlorange

MEdarkturquoise

MEblad

MEplum3

MElightcyan

MElightsteelblue1

ME avenderblush

MEoran gerede

MEantiqu en hite4

MEbluez

GSM1972937

60

1

GSM1972926

GSM1972928

GSM1972944

GSM1972919

GSM1972929

GSM1972938

GSM1972942

5

10

15

20

55

GSM1972920

GSM1972922

Soft threshold (power) Scale independence

1.0

50

45

3000

1

0.8

GSM1972925

GSM1972923

GSM1972940

GSM1972934

GSM1972930

GSM1972947

Mean connectivity

2000

0.6

0.4

OS time

1000

2

0.2

500

3

0

0.0

5 6 7 8 9 1011121314151617181820

5

10

15

20

Soft threshold (power)

C

Cluster dendogram

Network heatmap plot, selected genes

E

1.00-

0.95

0.90-

Height

0.85

0.80

0.75

0.70-

0.65

Dynamic tree cut

Merged dynamic

6

The original published paper had the same image for Figure 5E and 5F. Figure 5 should have been as follows:

F

G

H

GEO-COX

GEO-DEGS

774

255

144

MEnavajowhite1

MEbrown2

(63)

· Down Stable

-0.29

5

MEbrown4

-0.17

1.0

(0.4)

Up

25

6

MEdarkorange

0.27

MEdarkturquise

(0.2)

MEdarkgrey

0.067 (0.8)

0.2

0.5

4

302

MEblack

(0.4)

0.44

MEplum3

(0.02)

0.36

-log10 (p-value)

MElightcyan1

(0.03)

0.13 (0.6)

WGCNA-plum3

MElightsteelblue1

0.34 (0.1)

0.0

MEgreen

-0.29 (0.2)

2

Size of each list

MEmagenta

1059

-0.26

529.5

1095

MElavenderblush2

(0.2)

0.076

410

MEorangered4

(0.7)

-0.5

0

338

-0.28

TCGA-DEXs WGCNA-skyblue2

MEantiquawhite4

(0.2)

TCGA-COX

-0.47

MEblue2

(0.02)

0

Number of lements: specific (1) or shared by 2,3, . lists

-0.68

286

1220

(3e-04)

OS time

-1.0

-2

-1

0

1

2

3 (5) 2

1

log2 (fold change)

A

TCGA-ACC cohort

B

GSE76021 dataset

NDRG4 level +High+Low

CKS2 level

+

High+Low

NDRG4 level + High+Low

CKS2 level

+High+Low

1.00-

1.00

1.00-

1.00

Survival probability

0.75

Survival probability

0.75

Survival probability

0.75

Survival probability

0.75

0.50

0.50

0.50

0.50

0.25

p<0.001

0.25

p<0.001

0.25

p=0.002

0.25

p<0.001

0.00

0.00

0.00

0.00

0 1 2 3 4 5 6 7 8 9101112 Time (years)

0

1 2 3

4 1 5

6 7

8

9 1011 12

0 1 2 34 56 7 8 9 101112131415 16

0 12 3 4 5 6 7 8 9 101112 131415 16 Time (years)

Time (years)

Time (years)

C

Disease-free survival

D

Mitotane therapy

NDRG4 +High+Low

CKS2 +High+Low

NDRG4

+1 High+Low

CKS2

+

High-Low

1.00-

1.00

1.00-

1.00-

Survival probability

0.75

Survival probability

0.75

Survival probability

0.75

Survival probability

0.75

0.50

0.50

0.50

0.50

0.25

p<0.001

0.25

p<0.001

0.25

p<0.001

0.25

p<0.001

0.00

0.00

0.00

0.00

0

2

3

4

15678

9101112

0

1

2

3

4 5 6 7 8 9 101112

0 1 2 3 4 5 6 7 8 9101112 Time (years)

0

Time (years)

2 3 4 5 6 7 8 9 101112 Time (years)

Time (years)

E

F

3-year survival

5-year survival

100

100

80

80

Sensivity

60

Sensivity

60

40

40

AUC

20

AUC

P value

P value

Stage

0.804

reference

20

Stage

0.891

reference

CKS2

0.882

0.2091

CKS2

0.9763

NDRG4

0.906

0.1182

NDRG4

0.892

0.866

0.6718

0

Risk score

0.974

0.0016

0

Risk score

0.969

0.0313

0

20

40

60

80

100

0

20

40

60

80

100

100-specificity

100-specificity

ERRATUM

The formula on page 4 should read:

Riskscore=e^(B.+B +B *expression *expression )/(1+ e^(B.+B +B *expression +B )

TPstage(n) “PNDRG4

NDRG4 PCKS2

CKS2

tage(n) “PNDRG4

NDRG4 TPCKS2 * *expression CKS2

B represented regression coefficients. Expression represented the level of gene expression.

Reference:

Zhengqing Yang, Hui Cheng, Yazhou Zhang, Yan Zhou: Identification of NDRG Family Member 4 (NDRG4) and CDC28 Protein Kinase Regulatory Subunit 2 (CKS2) as Key Prognostic Genes in Adrenocortical Carcinoma by Transcriptomic Analysis. Med Sci Monit, 2021; 27: e928523. DOI: 10.12659/MSM.928523

Full-text PDF:

https://www.medscimonit.com/abstract/index/idArt/937786

This work is licensed under Creative Common Attribution- NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) e937786-3