35751045

A: No CDKN2C expression difference between various SCLC stages (III-IV vs. I-II).

CDKN2C mRNA Expression

GSE30219

CDKN2C mRNA Expression

GSE40275

8.5

NS

NS

0

12

8.0

0

7.5

11

7.0

0

O

10

6.5

6.0

9

5.5

I-II

III-IV

I-II

III-IV

Stage (sample n = 21)

Stage (sample n = 15)

B: Details of the predicted matched sequence between FOXA1 and CDKN2C.

Tool	TF	Motif ID	Sequence	Start	Stop	Strand	Scorea	p-value	Matched sequence
JASPAR	FOXA1	MA0148.4	chr1:50967831-50968826	30	41	+	7.25	|	TAGAAAACACTI
FIMOC	FOXA1	MA0148.4	chr1:50967831-50968826	30	41	+	6.48	0.001	TAGAAAACACTT

Notes: ªThe score for the motif occurrence; bJASPAR (2022); “Find Individual Motif Occurences (Version 5.4.1).

C: The seqlogo of FOXA1 motif within FOXA1 ChIP-Seq peak.

Additional file 3. CDKN2C expression difference between various SCLC stages (pane A), matched sequence of FOXA1 with CDKN2C (pane B), and FOXA1 motif (pane C).

1.5

GTAACAT

Bits

1.0

0.5

T

0.0

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