Effects of mitotane treatment on human steroid metabolism: implications for patient management
L Ghataore1, I Chakraborti2, S J Aylwin2, K-M Schulte2, D Dworakowska2,3, P Coskeran2 and N F Taylor1
Departments of 1Clinical Biochemistry, 2Medicine, King’s College Hospital, London SE5 9RS, UK 3Department of Endocrinology and Internal Medicine, Medical University of Gdansk, Gdansk, Poland
Endocrine CONNECTIONS
Correspondence should be addressed to N F Taylor Email norman.taylor1@nhs.net
Abstract
Mitotane (o,p’-DDD), an oral adrenolytic agent for treatment of advanced adrenocortical carcinoma (ACC), is reported to inhibit cortisol biosynthesis in vitro and enhance production from exogenous cortisol of urinary 66-hydroxycortisol and unidentified polar unconjugated metabolites. We examined urinary steroid profiles by gas chromatography-mass spectrometry of patients with histologically confirmed ACC following surgery, receiving a) hydrocortisone alone (three males and three females) and b) mitotane and hydrocortisone (six males and 11 females). Samples were collected after plasma mitotane had reached the therapeutic range of 14-20 mg/l. Increased excretion of polar unconjugated steroids during mitotane treatment was confirmed, with 6ß-hydroxycortisol and 60-hydroxy-20-dihydro- cortisols predominating. The proportion of additionally hydroxylated metabolites was <2% in untreated controls and 52, 35-52% (mean, range) in the mitotane plus hydrocortisone group. Ratios of 5x-/5ß- and 200-/20x-metabolites of administered cortisol were decreased 50-, 15-fold, and 14-, 8-fold respectively (males, females - mean values) but with no change in metabolite ratios that reflect oxidoreduction at C11 or C20. Patterns of decrease in 5g- relative to 5ß-reduced metabolites were similar to those of patients with
5a-reductase 2 deficiency or on treatment with the 5x-reductase 2 inhibitor finasteride but different from those of patients on dutasteride, indicating specific inhibition of 5g-reductase 2. We conclude that mitotane causes consistent changes in cortisol catabolismmost of which have not been previously recognised. These need not interfere with early detection of ACC recurrence. Induction of 6-hydroxylation offers an explanation for a reported decrease in cortisol bioavailability. Mitotane also has potential as a unique steroid metabolic probe for 20ß-reduction.
Key Words
steroid
cortisol
mitotane
GC-MS
Endocrine Connections (2012) 1, 37-47
Introduction
Mitotane (o,p’-DDD) plays an important role in adjuvant therapy of adrenocortical carcinoma (ACC) and in advanced stage disease (1). This application was first
reported in 1959 by Bergenstal et al. (2), but mitotane has only been in widespread use since publication in 2007 of the large retrospective study of Terzolo et al. (3).
@ 2012 The Authors. Published by BioScientifica Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Accumulated knowledge of the mode of action of mitotane together with its effects on steroid synthesis and catabolismis patchy.
Mitotane targets adrenocortical tissue but its precise mode of action is not fully understood. It requires metabolic activation via hydroxylation at the ß carbon (C2) followed by dehydrochlorination to form an acyl chloride (4). This binds covalently to mitochondrial proteins (4), causing selective adrenal toxicity via mito- chondrial destruction (5). Marked disruption of the zona fasciculata and reticularis but not glomerulosa has been shown in dogs (6). Cytotoxicity is also due to oxidative damage via free radical production (reviewed by Schtein- gart (7)). Dogs are the species most sensitive to mitotane. Human adrenal mitochondria have much lower capacity to both activate mitotane and bind its products and are thus less responsive (4).
Mitotane inhibits adrenocortical mitochondrial cholesterol side chain cleavage (CYP11A1) and 11B- hydroxylation (CYP11B1) activities in bovine adrenal tissue (8). Inhibition of CYP11B1 and 18-hydroxylation (CYP11B2) activities has been shown in slices of adrenal glands taken from patients treated with mitotane (9).
Patients with Cushing’s syndrome receiving mitotane have shown decrease in the adrenal cortisol secretion rate over time but a faster clinical benefit, suggesting that cortisol bioavailability is also diminished (10, 11). This has
been attributed to hepatic microsomal enzyme induction (8, 12) but the cortisol metabolic clearance rate, as estimated by classical techniques, is reported to be unchanged (10, 13). An increased dose requirement for hydrocortisone replacement during mitotane treatment is well established in clinical practice. Cortisol monitoring by standard methods is unhelpful due to mitotane- induced cortisol binding globulin increase and ACTH is more useful, with addition of free cortisol assay when patients have continuing Cushing’s syndrome (1).
Normal human cortisol catabolismtakes place primarily in the liver, with excretion into urine as glucuronide conjugates. These comprise (see box in Fig. 1) A-ring-reduced products 5ß-tetrahydrocortisone and 5g- and 5ß-tetrahydrocortisol; further reduction at C20 gives rise to the 20g- and 20ß-hydroxy products, cortolones and cortols. Cortisol and 6B-hydroxycortisol are mainly present in unconjugated form and together comprise <2% of the total products derived from cortisol.
The only reports of mitotane effects on extra-adrenal cortisol metabolism (10, 11, 13) note a rapid change in metabolism of administered cortisol after starting treat- ment, comprising a decrease in urinary tetrahydrocorti- sone, 5%- and 5ß-tetrahydrocortisol and a corresponding increase in excretion in an unconjugated polar steroid fraction, with 6B-hydroxycortisol as the only identified component.
Cortisol
Cortisone
118-HSD
30 HSD/ 5a-reductase
X
30+HSD/ 56-reductase
30-HSD/ 5,6-reductase
allo-Tetrahydrocortisol
Tetrahydrocortisol,
Tetrahydrocortisone
6₿-hydroxylase
200-HSD
20₿-HSD
60-hydroxylase
200-HSD
208-HSD
a-Cortol
₿-Cortol
a-Cortolone
₿-Cortolone
66-Hydroxycortisol
6a-Hydroxytetrahydrocortisol
200 HSD
20₿-HSD
68-Hydroxy-20a dihydrocortisol
66-Hydroxy-20-dihydrocortisol
arrows show pathways stimulated by mitotane. Names shaded white are for steroids that show decreases after mitotane.
We report here the results of urinary steroid profiling using a high-resolution capillary column for monitoring patients after removal of an ACC who were receiving mitotane combined with hydrocortisone (cortisol). We consistently found profound decreases in the common cortisol metabolites that are 5%- or 20ß-reduced and an increased excretion of several polar unconjugated cortisol metabolites. As hydrocortisone treatment is known to independently result in changes in urinary cortisol metabolite proportions compared with those derived from endogenous production, we took account of these by including a group of patients after removal of an ACC receiving only hydrocortisone, in addition to comparing with control data obtained from healthy volunteers. In order to seek to identify which 5a-reductase isoform was being targeted by mitotane, we also made comparisons with steroid excretion in patients with 5c-reductase 2 deficiency (5ARD2) and those receiving the 5g-reductase inhibitors finasteride or dutasteride.
Our findings of increase in polar cortisol metabolites further explain the observed increase in dose requirement in patients receiving hydrocortisone, while 5ARD2 inhi- bition would be predicted to diminish dihydrotestoster- one generation from testosterone, so that hypogonadal males might require additional androgen supple- mentation. Urinary steroid profile results obtained during monitoring for identified markers of ACC activity in patients after surgery can be appropriately interpreted in the light of this new knowledge.
Subjects and methods
Patients
The urine steroid data presented were obtained from samples submitted to our service for clinical purposes. Study groups comprised patients after surgery for ACC treated with mitotane (Lysodren, HRA Pharma, Paris, France) and oral hydrocortisone (six males: range 26-66 years, median 50 years and 11 females: range 20-76 years, median 47 years (six of premenopausal age)) and patients receiving hydrocortisone only after surgery in the period before mitotane was started (three males: 23, 54 and 65 years and three females: 37, 39 and 62 years). When there was more than one observation per patient, the median value was used. The mitotane dose was adjusted to maintain therapeutic concentrations of 14-20 mg/l and the hydrocortisone dose ranged from 20 to 50 mg/day. The data listed in Table 1 were obtained from patients in
whom plasma mitotane concentrations had reached the therapeutic range and who showed no evidence of ACC recurrence. In six patients, recurrence of ACC took place later in the monitoring period. All patients showed steroid profiles consistent with ACC before surgery, which was confirmed by histology of the removed mass (manuscript in preparation).
Other patient groups chosen for comparison had 5ARD2 deficiency (15 males: range 12-67 years, median 16 years and one female: 7 years) or were treated with finasteride, a specific inhibitor of 5ARD2 (14) (five males: range 42-77 years, median 42 years and one female: 40 years), or with dutasteride, an inhibitor of both 5ARD1 and 5ARD2 (three males: 66, 69 and 77 years).
The control group comprised healthy volunteers (20 males: range 22-49 years, median 31 years and 20 females: range 20-59 years, median 29 years).
Analytical procedures
Urinary steroid profiling was carried out according to our published method (15). In brief, steroids were extracted and conjugates were hydrolysed enzymatically using Helix pomatia digestive juice. The free steroid products were then re-extracted and methyl oxime-trimethylsilyl ether (MO-TMS) derivatives were prepared before analysis by gas chromatography-mass spectrometry (GC-MS) using a Perkin Elmer Clarus 500 system with an OV-1 column (Perkin Elmer, Beaconsfield, Buckinghamshire, UK). Quantification was based on data obtained in cyclic scan mode. Additional procedures were carried out using published protocols for steroid conjugate separation on Sephadex LH-20 (15), solvolysis (16) and sodium borohy- dride reduction (17). Steroid ratios were compared using one-way ANOVA after logarithmic transformation followed by post hoc comparisons by the method of Bonferroni.
Results
Figure 1 summarises the overall effects of mitotane on cortisol catabolismas a background to the findings described in detail below. Figure 2 shows examples of single urinary steroid profiles obtained by GC-MS to illustrate differences between post-surgery ACC patients receiving hydrocortisone with and without mitotane and healthy volunteers. Table 1 lists the cortisol metabolites identified, together with quantitative data based on analyses in all patients.
| Non polar steroids | Normal | Hydrocortisone + mitotane | ||
|---|---|---|---|---|
| Percentage of mean (range) | Percentage of mean (range) | |||
| Male (n=20) | Female (n=20) | Male (n=6) | Female (n=11) | |
| Tetrahydrocortisone | 39 (28-55) | 39 (28-45) | 35 (30-38) | 28 (18-40) |
| 56-Tetrahydrocortisol | 17 (11-25) | 18 (12-24) | 43 (37-53) | 45 (35-56) |
| 5a-Tetrahydrocortisol | 15 (6-27) | 11 (5-26) | 0.6 (0.1-0.8) | 1.7 (0.1-7) |
| B-cortolone | 6.9 (7-8) | 7.3 (4-11) | 0.9 (0.2-1.6) | 1.5 (1-4) |
| B-cortol | 2.3 (2-3) | 2.4 (1-4) | 0.3 (0.1-0.5) | 0.5 (0.2-1.2) |
| a-cortolone | 8.7 (6-2) | 11 (8-16) | 14 (9-16) | 14 (9-19) |
| a-cortol | 2.6 (1-4) | 2.6 (1-4) | 6.0 (4-8) | 9.2 (4-19) |
| Polar steroids | Male (n=20) | Female (n=20) | Male (n=4) | Female (n=4) |
|---|---|---|---|---|
| 6ß-Hydroxycortisol | <2 | <2 | 32 (30-39) | 28 (12-37) |
| 6ß-Hydroxy-20a-dihydrocortisol | <2 | <2 | 10 (7-17) | 10 (3-18) |
| 6ß-Hydroxy-20ß-dihydrocortisol | <2 | <2 | 4.1 (2-8) | 3.6 (1-8) |
| 6a-Hydroxytetrahydrocortisol | <2 | <2 | 5.5 (3-8) | 4.9 (3-6) |
| 6a-Hydroxy-a-cortolone | <2 | <2 | 1.6 (1.3-2.2) | 1.5 (0.2-2.7) |
| 6a-Hydroxy-B-cortolone | <2 | <2 | 0.1 (0-0.3) | 0.06 (0-0.1) |
| 1ß-Hydroxy-a-cortolone | <2 | <2 | 1.7 (0.9-3.1) | 2.3 (0.8-3.3) |
| 16-Hydroxy-ß-cortolone | <2 | <2 | 1.6 (0.9-2.6) | 2.4 (0.6-4.4) |
1B- and 6-Hydroxylation
It was evident that there were large increases in polar (late-eluting) cortisol metabolites after mitotane. These were mostly 6-hydroxylated, but smaller amounts of 1ß-hydroxylated metabolites also became detectable. None of these were initially apparent during analysis by our standard method, which involves a wash step in which the sample is partitioned between water and ethyl acetate. Omitting this step led to much higher recoveries, showing that the steroids were being lost in the water phase and confirming early observations (10) that a large proportion of cortisol metabolites is found in a polar fraction after mitotane treatment.
Following chromatography of the urine extract on Sephadex LH-20, the polar metabolites were all found in a ‘free plus glucuronide’ fraction (15). None were found in a sulphate fraction. In case there were sulphates conjugated at positions that were resistant to Helix pomatia hydrolysis,
a chemical cleavage procedure, solvolysis, was also carried out on this fraction. This yielded no additional steroid products. Formation of MO-TMS derivatives of urine extracts before and after Helix pomatia hydrolysis gave similar yields of the polar metabolites, showing that nearly all were present as free steroids.
The largest component was 6B-hydroxycortisol. Four polar steroid peaks gave similar mass spectra, interpreted as being 6ß-hydroxy-20-dihydrocortisols. Their retention times corresponded to 20%- and 20ß- epimers, with each giving rise to syn/anti pairs of the 3-MO. Identification was confirmed by their sodium borohydride reduction products being identical with those obtained by reduction of standard 6ß-hydroxycortisol. Small amounts of A-ring- reduced metabolites included 6%-hydroxytetrahydrocorti- sol, identified by comparison with published data (18) and 1B- and 6x-hydroxycortolones, which matched steroids we have identified in newborns (19). In order to examine the extent of 1B- and 6-hydroxylation a subgroup of
5ß-THF
Scan El+
100
(a)
Patient receiving mitotane & hydrocortisone
6
3.78×10°
1
%
A
THE
5a-THF
3
8
20a
5
B
6
8c
2/4
7
0
Relative abundance (%)
100
(b) Patient receiving hydrocortisone
56-THF
20₿
20a
7
Scan El+
A
2.85×109
THE
20₽
5a-THF
20a
%
200
₩/ 20a
B
C
0
(c) Normal subject
THE
Scan El+
100
2.48×10°
A
50-THF
%
5a-THF
20a 208
20a
B
C
0
18.00
23.00
28.00
33.00
38.00
Time (min)
Figure 2 Total ion current chromatograms from GC-MS analysis of urinary steroid metabolites excreted (a) by a patient after surgery when receiving mitotane and hydrocortisone, (b) the same patient on a previous occasion when receiving hydrocortisone only and (c) a normal subject. A, B, C, internal standards: androstanediol, stigmasterol and cholesterol butyrate; THE, tetrahydrocortisone; THF, tetrahydrocortisol (5x- and 5ß-reduced epimers shown); 20g- and 20ß-, 20-reduced steroids in the order a-cortolone, B-cortol, B-cortolone, a-cortol. Numbered peaks (repeat numbers show syn/anti-pairs of the methyl oxime derived from a single
samples was analysed without the wash step (Table 1, lower panel). For these, 52% (range 35-52) of total recovered cortisol metabolites were 1 or 6-hydroxylated compared with <2% in controls. Figure 3a shows detection by selected ion monitoring MS of cortisol and its major polar products.
5a-Reduction
A profound decrease in 5g-reduced steroid metabolites relative to their 5ß-reduced counterparts was seen in patients receiving mitotane (Figs 2, 3b and c). Figure 4a shows the ratios for 50/50-tetrahydrocortisol for controls and five patient groups. These are divided between males and females to take account of known gender differences (20). Hydrocortisone treatment was associated with lower values, as expected (21) (but not significant), while for the mitotane and hydrocortisone group, there was a marked fall in the ratio compared with control (P<0.001 (both genders, data for separate genders given in the following
steroid) are 1, 6x-hydroxytetrahydrocortisol; 2, 6x-hydroxy-a-cortolone; 3, 1B-hydroxy-a-cortolone; 4, 6%-hydroxy-B-cortolone; 5, 10-hydroxy-ß- cortolone; 6, 66-hydroxycortisol; 7, 60-hydroxy-20-dihydrocortisol and 8, 6-hydroxy-20c-dihydrocortisol. Samples were prepared without a wash step (see Results section) to provide good recovery of polar cortisol metabolites. The steroids marked were identified by comparison of mass spectra and retention times with those of authentic standards, or as detailed in the Results section.
paragraphs if different) and hydrocortisone-only groups (P<0.001)). There were no significant differences between the mitotane and hydrocortisone group and the remaining groups.
It was noticeable (Fig. 4b) that for the androstene- dione metabolites androsterone (5a-reduced) and aetio- cholanolone (5ß-reduced), the degree of decrease in 5%. reduction was not as profound as for cortisol. This mirrors findings in 5ARD2 deficiency (22). There was no change following hydrocortisone treatment, while for the mito- tane and hydrocortisone group, there was a fall in the ratio compared with control in males (P<0.001) but not in females. Indeed, after mitotane, nine females individually showed an increase in the ratio of androsterone/aetiocho- lanolone relative to their pre-mitotane values, with single pre- and post-menopausal patients showing falls. In contrast, in all males (n=6), the ratio decreased after mitotane was started. There were no significant differences between the mitotane and hydrocortisone group and the remaining groups except for dutasteride, which is known
Mitotane & hydrocortisone
6B-Hydroxy-20a-dihydrocortisol
100
6B-Hydroxy-20B-dihydrocortisol
m/z
384
Relative abundance (%)
0
100
6ß-Hydroxycortisol
5
693
0
100
Cortisol
M
605
0
100
À
6a-Hydroxytetrahydrocortisol
740
0
100
TIC
0
30.70
32.70
34.70
36.70
38.70
Time (min)
(b) Normal
(c) Mitotane & hydrocortisone
m/z
100
cortol
20g
1
253
M
20฿
20g
20B
0
Relative abundance (%)
100
cortolone
20a
20g
1
449
20฿
0
tetrahydrocortisol
58 5x
M
5₿
100
11
5a
652
0
100
TIC (total ion current) chromatogram
TIC
0
28.50
29.50
30.50
31.50
32.50
28.50
29.50
30.50
31.50
32.50
Time (min)
Figure 3 Selected ion chromatograms from GC-MS analysis of urine from a patient receiving mitotane and hydrocortisone in comparison with an untreated subject. Plots of selected ions (m/z) and total ion current (TIC) shown are for (a) cortisol and its major 6-hydroxylated metabolites in the patient
(where two peaks are marked, these are the syn/anti-pairs of the methyloximes of a single steroid), (b) 5g- and 5- and 20g- and 200-reduced pairs of cortisol metabolites for the untreated subject and (c) the same ions for the patient.
to inhibit both 5ARD1 and 5ARD2, where the ratio was lower (P<0.001, compared in males only). This leads to the conclusion that the remaining generation of androsterone relies on the activity of 5ARD1, in contrast to 5g-tetrahydrocortisol generation, which entirely depends on 5ARD2. Mitotane thus appears to specifically inhibit 5ARD2.
All female patients of premenopausal age showed markedly low urinary excretion of androsterone (ug/24 h, mean 111, range 27-255) and aetiocholanolone (mean 165, range 16-222) in comparison with the control group (androsterone (mean 746, range 335-1483, P=0.002) and aetiocholanolone (mean 865, range 350-1620, P=0.002)). Changes in serum progesterone and urinary pregnanediol, quantified on several occasions in two of the women of reproductive age, indicated that both were cycling. One reported regular bleeding and the other had previously had a hysterectomy. In contrast, male patients showed decrease in androsterone but not in aetiocholanolone.
20ß-Reduction
A decrease in the 20ß-reduced cortisol metabolites relative to their 20c-reduced counterparts was seen in patients receiving mitotane and hydrocortisone (P<0.001, Figs 2, 3b and c, 5a). This was most profound for cortols, cortolones and 6x-hydroxycortolones and less marked for 6B-hydroxy-20-dihydrocortisols and 16-hydroxycorto- lones (Table 1).
Other cortisol metabolism
Figure 5b, c and d shows cortisol metabolite ratios that express the effects of mitotane other than on 5g- and 200-reduction. Figure 5b and c shows that there are no net changes in oxidoreduction at C11 and C20, respectively, indicating that there are no suppressive effects on 11- or 20-hydroxysteroid dehydrogenase activities. Figure 5d shows that increased excretion of 60-hydroxy steroids occurs only in the hydrocortisone and mitotane group.
(a)
5a/5ß-tetrahydrocortisol
1.2
T=20
1
Male Fem ale
0.8
n=20
n=3
Ratio
0.6
0.4
FF3
0.2
n=6
== 11
n=15 m=1
0
n=5
/F1
n=3
Control
Hcort
Hcort+Mit
5ARD2
Finasteride
Dutasteride
(b)
Androsterone (5a)/Aetiocholanolone (56)
2.5
2
Ratio
1.5
1
0.5
0
Control
Hcort
Hcort+Mit
5ARD2
Finasteride
Dutasteride
Effect on ACC markers
All patients who had had a recurrence of ACC during mitotane treatment showed steroid excesses before surgery, which included secretion of 11-deoxycortisol, with 20g- and 20ß epimers of hexahydro-11-deoxycor- tisols being excreted in similar amounts. On recurrence, the 20ß-reduced epimer was much diminished, as expected. Other major markers showed unchanged metabolism. These were (manuscript in preparation) pregnanediol (20%), pregnanetriol (20%), DHEA, DHEA metabolites, tetrahydro-11-deoxycortisol (5฿) and 5-pregnene-30,16%,20c-triol. This was also expected, as none of these steroids are 5x- or 200-reduced.
Dose-response
No metabolic changes were found to correlate with either dose or blood concentrations of mitotane when these were within the therapeutic range. However, when blood mitotane was monitored at intervals in a single female patient during an 18 month period following withdrawal of treatment due to neurological complications, a dose- response relationship for inhibition of 5%- and 20ß- reduction was evident (Fig. 6). It can be seen that complete inhibition is present for both enzymes at a threshold plasma mitotane concentration much lower than the therapeutic range, and this would explain the lack of
correlation noted earlier. The dose response for 6ß- hydroxylation was unfortunately not obtained because no material remained for analysis by the method modified to recover very polar steroids. However, in a few patients from whom single samples were taken before plasma mitotane concentrations had attained the therapeutic range, all steroid metabolite ratios were as low as they were in later samples (data not shown).
Discussion
This study shows that mitotane treatment in patients with ACC results in consistent, but mostly previously unrecog- nised, changes in cortisol metabolism. These comprise suppression of 5g- and 20ß-reduction, together with induction of 16- and 6-hydroxylation, as summarised in Fig. 1. On limited data, these changes appear to be essentially complete at plasma concentrations of mitotane that are much lower than the therapeutic range.
The urinary 6-hydroxylated metabolites are confirmed as being present at relatively very high concentrations and in unconjugated form. Rapid improvement in patients with Cushing’s syndrome (10, 11) and increased hydro- cortisone dose requirement during mitotane therapy (1) may thus be explained by decreased bioavailability of oral cortisol due to rapid conversion by CYP3A4 in the liver, perhaps during a first pass after adsorption from the gut. A second factor may be an increase in corticosteroid binding globulin during mitotane treatment (23).
Induction of CYP3A4 by mitotane needs to be considered when selecting drugs and drug dose levels. Kroiss et al. (24) have provided a comprehensive list of those likely to be affected. Oral bioavailabilities of the tyrosine kinase inhibitor sunitinib and the anticonvulsant midazolam are profoundly decreased at a plasma mitotane range of 4.9-17.8 mg/l (25).
Polar 1B- and 6x-hydroxylated cortisol metabolites are important components in urine from newborns (19), but the pathways of their formation have not yet been studied. A close relationship between them (Taylor NF, 2012, unpublished observations) suggests that a single enzyme is responsible for both the additional hydroxylations. This might also be CYP3A4, with saturation of the A-ring directing formation of a 6x-hydroxyl and unsaturation directing formation of the better known 6ß-hydroxyl, but induction by mitotane of additional cytochromes is also possible.
Mitotane has a specific cytotoxic effect on the mitochondria of adrenal cortical cells and produces focal degeneration of the reticularis and fasciculata zones and
(a)
20ß/20a-reduced cortisol metabolites
1
0.9
n=20
0.8
T
n=20
n=3
0.7
0.6
n=3
Ratio
T
0.5
0.4
0.3
0.2
n=11
0.1
n=6
0
Control
Hcort
Hcort+Mit
(c) 20oxo/20OH-reduced cortisol metabolites
6
5
4
Ratio
3
T
2
1
0
Control
Hcort
Hcort+Mit
only small changes in the zona glomerulosa (6). However, evidence for decrease in cortisol secretion due to mitotane as a contributor to increased hydrocortisone dose require- ment is lacking. In a single patient with secondary Cushing’s syndrome, short-term treatment did not dimin- ish cortisol metabolic clearance rate nor secretion rate (10). During long-term administration to six patients with various clinical disorders, cortisol secretion rate was diminished in two, did not change in two and increased in two (11). This might be explained by variable compensation by hypothalamusry-adrenal axis activation.
In contrast, a larger decrease in androgen metabolites post-mitotane in the females than males in this study may more clearly reflect adrenal damage, as there is a greater contribution of the adrenal cortex to these metabolites in females (around 50 vs 30%) (26) and decreased androgen synthesis in the male may be compensated by LH increase. However, the very low androgen metabolite excretion in the females, even in those of premenopausal age, suggests an additional affect on ovarian androgen secretion. As this arises primarily from the theca cells, it is remarkable that
(b)
11oxo/11OH-reduced cortisol metabolites
2
1.8
1.6
1.4
Ratio
1.2
1
0.8
0.6
0.4
0.2
0
Control
Hcort
Hcort+Mit
(d) 60H/Total cortisol metabolites
2.5
2
Ratio
1.5
1
0.5
0
Control
Hcort
Hcort+Mit
B-cortolones + a- and B-cortols and (d) 6x-hydroxytetrahydrocortisol + 6ß-hydroxycortisol +66-hydroxy-20g- and 20ß-dihydrocortisol/total cortisol metabolites. Numbers in each group are as shown in (a); bars are for means, with standard errors marked.
cycling was seen to continue in the two treated women on whom we had information.
Our findings of previously unreported effects on cortisol 5- and 20-reduction rely on resolution by GC- MS of individual cortisol metabolites. Early studies would have missed the decrease in 5x-tetrahydrocortisol because the celite chromatographic method used did not resolve it from tetrahydrocortisone (10), while the 20-reduced cortolones and cortols were not reported. The effect on 5-reduction has recently been reported in abstract form (27), in a study also based on GC-MS analysis.
Our interpretation that mitotane probably specifically inhibits 5ARD2 assumes that decrease in the ratio of 5x./5B- reduced metabolites reflects only decrease in 5a-reductase activity, but both mitotane (28) and finasteride (29) also inhibit 5ß-reductase (AKR1D1); we could not locate any reports of an effect of dutasteride. This could contribute to the smaller change in these ratios during mitotane and finasteride than during dutasteride. The increase in the ratio of androsterone/aetiocholanolone post-mitotane in females but not in males represents a numerically small change but may reflect a gender difference in balance of
20₿/20a
1.4
1.2
Ratio
1
0.8
0.6
0.4
0.2
14-20 mg/l
0
-5
0
5
10
15
20
25
Plasma mitotane levels (mg/l)
1.4
5a/5ß-tetrahydrocortisol
1.2
Ratio
1
0.8
0.6
0.4
0.2
14-20 mg/l
0
0.2
-5
0
5
10
15
20
25
Plasma mitotane levels (mg/l)
gonadal 5-reductases. A third 5a-reductase has recently been reported in prostate cancer cell lines (30). Inhibition of an unrecognised ovarian 5ß-reductase is also possible. This enzyme class is widely distributed in vertebrates (31). In a single female patient with documented AKR1D1 deficiency, there was a decrease in 5ß-reduced urinary androgen metabolites to 20% of normal (32) but near absence of 5ß-tetrahydrocortisol. This could be taken as evidence for the presence of another, unaffected, 5ß-reductase isoform.
The 5a-reductase isoform, 5ARD2, which is required for hepatic cortisol metabolism, is the same as that required for generation of dihydrotestosterone from testosterone in androgen target tissues. This is evident from consistent diagnostic decreases in the ratio of urinary 5x/5ß-tetrahydrocortisol in patients with 5ARD2
deficiency (22), although a recent review (33) concluded, surprisingly, that hepatic cortisol reduction used 5ARD1, based on in vitro findings. Inhibition of 5ARD2 by mitotane could explain our clinical observations that hypoandrogenic male patients on testosterone replace- ment tend to remain hypoandrogenic at standard doses when also receiving mitotane. This could be explored by measurement of the serum testosterone:dihydro- testosterone ratio or of serum androsterone glucuronide as a marker of peripheral dihydrotestosterone production, but we have not attempted this. Mitotane increases hepatic production of sex hormone binding globulin (SHBG) (23), so this may be another factor. Trial of the androgen analogue, nandrolone (19-nortestosterone), for androgen replacement would be worthwhile. This steroid is rendered less androgenic by 5a-reduction to dihydro- nandrolone (34), so would be expected to be made more potent by mitotane.
Variable effects of mitotane on 20ß-reduction accord- ing to substrate may reflect differential effects on substrate binding by a single oxidoreductase or on several. Steroid reductases frequently have wide specificity. While it has been recognised that both AKR1C1 and AKR1C3 show 20%-reductase activity, no enzymes with 20ß-reducing activity have yet been defined to our knowledge, even though this reaction in liver is quantitatively important in corticosteroid catabolismoxidoreductases for C5 and C20 positions are potentially important as regulators of neurosteroid formation. Mitotane offers the first specific probe for 20ß-reduction activity.
It may be concluded that these studies support and expand previous findings, emphasising that mitotane has profound effects on metabolism of cortisol of exogenous origin, and this would diminish oral bioavailability and further activation. The effects on steroid metabolism take place at concentrations that are below the therapeutic range and so could not provide a surrogate for plasma mitotane assay for monitoring capacity for adrenocortical tumour suppression. However, a finding of the charac- teristic steroid profile changes in patients in whom plasma mitotane concentrations are low, such as when they have only recently commenced mitotane or have ceased treatment and clearance is continuing, might provide a useful reminder that effects on drug dynamics are still likely.
Mitotane in its bioactivated form has potential as a tool for the study of enzymes involved in steroid activation and catabolismincluding 20-reduction activity, for which no specific probes have been previously recognised.
Declaration of interest
The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
Funding
This work was wholly supported from internal resources.
Author contribution statement
Part of this work has been presented in abstract form (35, 36). Mrs L Ghataore carried out most of the analytical work, wrote text and prepared figures, Dr I Chakraborti reviewed and collated clinical and mitotane concentration data, Dr S Aylwin gave permission for study of patients under his care and provided clinical insights, Mr K-M Schulte gave permission for study of patients under his care on whom he had performed surgery and provided clinical insights, Dr D Dworakowska reviewed and collated clinical and mitotane concentration data and provided clinical insights, Mrs P Coskeran recorded mitotane concentrations and other data relating to nursing care during patient visits and Dr N F Taylor carried out some of the analytical work, wrote text and prepared figures.
Acknowledgements
The authors wish to thank Sophie Guerinet and Rita Chadarevian (HRA Pharma, Paris, France) for helpful comments on the manuscript, the Lysodren service of HRA Pharma for plasma mitotane analysis, Dr Angel Chan, Queen Elizabeth Hospital, Hong Kong, for her expert review of the manuscript and Charles West for help with calculation of data.
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Received in final form 7 June 2012 Accepted 11 June 2012
@ 2012 The Authors. Published by BioScientifica Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.